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Triplets, radical cations and neutral semiquinone radicals of lumiflavin and riboflavin: An overhaul of previous pump–probe data and new multichannel absolute absorption spectra

Paper ID Volume ID Publish Year Pages File Format Full-Text
30829 44507 2012 6 PDF Available
Title
Triplets, radical cations and neutral semiquinone radicals of lumiflavin and riboflavin: An overhaul of previous pump–probe data and new multichannel absolute absorption spectra
Abstract

Though long-lived transient photoproducts of flavins have received much attention over the last five decades, their spectroscopic characterization remains rudimentary and altogether inconclusive. Lumiflavin and riboflavin have therefore been reexamined, using nanosecond laser photolysis and multichannel detection over a wide spectral range (230–900 nm); differential absorption spectra of flavin solutions in aqueous buffer at neutral pH have been recorded; radical cations and semiquinone radicals were generated by quenching the triplets with C (NO2)4 (electron acceptor) and NO2- (electron donor), respectively. The problem of estimating the extent of ground state depletion after a single flash has been solved by developing a new strategy that provides reliable lower and upper bounds for the depletion (and for the molar absorption coefficients of the transient species of interest). The spectra of the neutral radicals and triplets of the two flavins are almost identical, but those of the two cations are not.

Graphical abstractA consistent set of absolute absorption spectra of F†, F•+ and FH• are presented (F = lumiflavin or riboflavin).Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Flavin photochemistry; Flash photolysis
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Triplets, radical cations and neutral semiquinone radicals of lumiflavin and riboflavin: An overhaul of previous pump–probe data and new multichannel absolute absorption spectra
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 106, 5 January 2012, Pages 34–39
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us