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Protective effect of triphlorethol-A against ultraviolet B-mediated damage of human keratinocytes

Paper ID Volume ID Publish Year Pages File Format Full-Text
30835 44507 2012 7 PDF Available
Title
Protective effect of triphlorethol-A against ultraviolet B-mediated damage of human keratinocytes
Abstract

Ultraviolet B (UVB) radiation on human skin induces pathophysiological processes such as oxidative stress and inflammation. In previous reports, the antioxidant effects of triphlorethol-A were shown to protect cells against hydrogen peroxide-induced cell damage and gamma ray-induced oxidative stress. In this study, the role of triphlorethol-A in protecting human keratinocytes (HaCaT) against UVB-induced cell damage was investigated. Triphlorethol-A-treated cells were irradiated with UVB (150 mJ/cm2). Triphlorethol-A decreased UVB-induced intracellular ROS and restored the activities of antioxidant enzymes decreased by UVB radiation. Triphlorethol-A decreased UVB damage to cellular components, such as lipid membrane and DNA, restored cell viability and reduced UVB-induced apoptosis by inhibiting the mitochondria-mediated caspase pathway. Triphlorethol-A also reduced the UVB-induced loss of ΔΨm and the active forms of caspase 9 and caspase 3. The anti-apoptotic effect of triphlorethol-A was found to involve the inhibition of c-Jun NH2-terminal kinase, which was induced by UVB exposure. And triphlorethol-A showed an absorptive capacity at range of UVB.These results suggest that triphlorethol-A protects human keratinocytes against UVB by enhancing the activities of the antioxidant system, inhibiting cellular damage and absorbing the UVB.

► Triphlorethol-A decreased UVB-induced intracellular ROS. ► Triphlorethol-A restored the activities of antioxidant enzymes decreased by UVB. ► Triphlorethol-A decreased UVB damage to cellular components and apoptosis. ► Triphlorethol-A protected human keratinocytes against UVB-induced oxidative stress.

Keywords
Triphlorethol-A; Ultraviolet B; Oxidative stress; Reactive oxygen species; Human keratinocytes; Apoptosis
First Page Preview
Protective effect of triphlorethol-A against ultraviolet B-mediated damage of human keratinocytes
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 106, 5 January 2012, Pages 74–80
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering