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Effect of pH on the interaction of baicalein with lysozyme by spectroscopic approaches

Paper ID Volume ID Publish Year Pages File Format Full-Text
30847 44508 2011 11 PDF Available
Title
Effect of pH on the interaction of baicalein with lysozyme by spectroscopic approaches
Abstract

The interaction mechanism of baicalein and lysozyme (Lys) has been characterized by fluorescence, synchronous fluorescence, ultraviolet–vis absorbance, and three-dimensional (3D) fluorescence. The structural characteristics of baicalein and Lys were probed, and their binding affinities were determined under different pH conditions (pH 7.4, 4.5, and 2.5). The results showed that the binding abilities of the drug to Lys increased under lower pH conditions (pH 4.5 and 2.5) due to the alterations of the protein secondary and tertiary structures or the structural change of baicalein. The effect of baicalein on the conformation of Lys was analyzed using UV, synchronous fluorescence and three-dimensional (3D) fluorescence under different pH conditions. These results indicate that the binding of baicalein to Lys causes apparent change in the secondary and tertiary structure of Lys. In the presence of Cu2+, the decrease of the binding constant in buffer solution of pH 2.5 may result from the competition of the metal ion and baicalein binding to Lys. In addition, the presence of Cu2+ increased the binding constants of baicalein–Lys complex under higher pH conditions (pH 7.4 and 4.5). The possible site of binding of baicalein to Lys has been proposed to explain these observations.

► The intrinsic fluorescence of Lys was quenched by the addition of baicalein at pH 2.5, 4.5, and 7.4. ► The effect of Cu2+ on the interaction of baicalein with Lys was studied with various pH values. ► Binding affinities were determined under different pH conditions (pH 2.5, 4.5, and 7.4). ► The effect of baicalein on the conformation of Lys was analyzed. ► The possible site of binding of baicalein to Lys has been proposed.

Keywords
Baicalein; Lysozyme; Fluorescence quenching; Binding constant; Three-dimensional fluorescence
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Effect of pH on the interaction of baicalein with lysozyme by spectroscopic approaches
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 104, Issue 3, 2 September 2011, Pages 414–424
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us