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The biological effects of 632.8-nm low energy He–Ne laser on peripheral blood mononuclear cells in vitro

Paper ID Volume ID Publish Year Pages File Format Full-Text
30877 44511 2006 4 PDF Available
Title
The biological effects of 632.8-nm low energy He–Ne laser on peripheral blood mononuclear cells in vitro
Abstract

The aim of this study was to examine the proliferation of peripheral blood mononuclear cells due to the low energy 632.8-nm He–Ne laser application. The results of previous studies supported the hypothesis that low level laser therapy (LLLT) might have an increasing effect on the proliferation of lymphocytes and production of cytokines. The effect of laser irradiation was investigated by comparing the proliferation of peripheral blood mononuclear cells (PBMC) with a mitogenic stimulator, PHA (phytohemaglutinin) and laser irradiation. PBMCs of control samples, only laser irradiated samples, PHA included samples and both PHA included and laser irradiated samples were quantified and compared. Results of [3H] Thymidine test, 20 s laser irradiated and 40 s laser irradiated samples’ proliferation were found statistically higher than control samples. There was no significant difference between control and 60 s laser irradiated samples. PHA also showed its ability to proliferate cells. PHA included samples and both PHA included and laser irradiated samples’ proliferation was higher than both control and only laser irradiated samples. Our results showed that He–Ne laser application enhanced the proliferation significantly. Moreover, laser dose was noted as a significant parameter. On the other hand, LLLT by itself was found less effective than PHA.

Keywords
Biostimulation; Blood cells; He–Ne laser; PHA
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The biological effects of 632.8-nm low energy He–Ne laser on peripheral blood mononuclear cells in vitro
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 82, Issue 3, 1 March 2006, Pages 199–202
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us