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Age-dependent DNA repair and cell cycle distribution of human skin fibroblasts in response to UVA irradiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
30880 44511 2006 10 PDF Available
Title
Age-dependent DNA repair and cell cycle distribution of human skin fibroblasts in response to UVA irradiation
Abstract

Ageing process in cells is associated with oxidative stress. Ultraviolet A produces reactive oxygen species responsible for accumulation of DNA and cellular damage. After the evaluation of antioxidant enzyme activities and oxidative stress markers at the basal state, we have studied the responses to UVA stress of coetaneous fibroblasts, isolated from different male donors (2–88 years, n = 23) in terms of cytotoxicity, genotoxicity and DNA repair capacities. For this purpose, we have determined level of DNA damage using the comet assay (single strand breaks and alkali-labile sites) and the cell cycle distribution after a 5 J/cm2 irradiation.No differences with age were observed for antioxidant enzyme activities and oxidative stress markers. DNA strand breaks after UVA irradiation (5–20 J/cm2), was found to be age-dependent. DNA repair was slow and also significantly affected by ageing. The cell cycle distribution analysis showed that high repair correlated with high proliferative capacities at basal level. Twenty-four hours after the stress, fraction of young fibroblasts blocked in G1 phase was significantly increased whereas significant modifications concerned the G2-M phase for adult and older fibroblasts. These results indicate an age-dependent decline in the DNA repair capacities correlated with modifications of the cell cycle parameters.

Keywords
Ageing; Oxidative stress; DNA damage; Comet assay
First Page Preview
Age-dependent DNA repair and cell cycle distribution of human skin fibroblasts in response to UVA irradiation
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 82, Issue 3, 1 March 2006, Pages 214–223
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering