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High sensitivity biosensor measurement based on synchronous detection

Paper ID Volume ID Publish Year Pages File Format Full-Text
30888 44512 2011 8 PDF Available
Title
High sensitivity biosensor measurement based on synchronous detection
Abstract

The principle of synchronous detection (SD) has been applied to biosensor measurement. SD principle achieves significant increases in the signal to noise ratio, limit of detection and overall measurement robustness. Application of SD in biosensor measurement improves the analysis of the response and avoids the influence of interference/noise produced by stirring, electromagnetic effects and influence of parasitic currents. SD also enables the decomposition of signal to stimulation response and phenomena with long time of response. Second-order phenomena are identifiable in the signal. Linear statistical model was used to develop software for identification of the stimulation signal in the output current. SD was applied to the response signal of a Photosystem II complex (PSII) biosensor. PSII response to light stimulation follows first order kinetics. The inhibition kinetics of PSII has been studied. Kinetic constants of herbicide binding to PSII depend linearly on herbicide concentration and enable measurement of its concentration at low concentrations (linear range for diuron is 10−6 to 10−4 mM).

Keywords
Biosensor; Herbicides; Inhibition; Photosystem II; Synchronous detection
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 102, Issue 3, 2 March 2011, Pages 192–199
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us