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Quantum dots/particle-based immunofluorescence assay: Synthesis, characterization and application

Paper ID Volume ID Publish Year Pages File Format Full-Text
30905 44513 2009 6 PDF Available
Title
Quantum dots/particle-based immunofluorescence assay: Synthesis, characterization and application
Abstract

Recently, it has been proved that quantum dots (QDs) hold the potential to be used in the bioanalysis as fluorescent probes for their many unique optical properties. In this paper, immunofluorescence assay, an integration of particle-based immunoassays and fluorescent QD-probes, was constructed. Firstly, high quality CdSe/ZnS QDs were prepared. Then after being water-solubilized by amphiphilic polymer based on self-assembling, the QDs were labeled by immunoglobulin G (IgG) antibody. At the same time, both carboxyl-polystyrene (PS) and magnetic carboxyl-PS microspheres were prepared and coated by antigens. The antigen sensitized PS microspheres were specifically captured by the QD–IgG bioconjugates based on the antibody-antigen reaction, which was confirmed by the immunofluorescence test in vitro. The sensitivity of current assay was tested by sandwich immunofluorescence assay using human alpha fetoprotein (AFP) as antigen model. The detection limit of AFP antigen is 4.9 ng/mL.

Keywords
Quantum dots; Polystyrene microspheres; Magnetic; Fluorescent; Immunofluorescence assays
First Page Preview
Quantum dots/particle-based immunofluorescence assay: Synthesis, characterization and application
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 94, Issue 1, 9 January 2009, Pages 45–50
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering