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In vitro photodynamic inactivation of Candida spp. growth and adhesion to buccal epithelial cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
30909 44513 2009 6 PDF Available
Title
In vitro photodynamic inactivation of Candida spp. growth and adhesion to buccal epithelial cells
Abstract

In this study, photodynamic inactivation (PDI) was used to inhibit in vitro growth and adhesion of different Candida isolates to buccal epithelial cells (BEC). Experimental conditions were optimized and 25 μM toluidine blue O (TBO) and 15 min of irradiation time by light emitting diode (LED) (energy density of 180 J/cm2) were selected due to higher reductions in cellular viability obtained after treatment. Reduction media of Log10 3.41 in viable cellular growth and media of 55% in the inhibition of adhesion to buccal epithelial cells were obtained. Two fluconazole resistant isolates were susceptible to PDI (Log10 3.54 in IB05 and Log10 1.95 in CG09) and a second session of this treatment for CG09 isolate inhibited cellular viability in 100%, without producing heat. The results permit to conclude that photodynamic inactivation under these experimental conditions would be a possible alternative approach to inhibit Candida spp. cellular growth and adhesion to buccal epithelial cells.

Keywords
Photodynamic inactivation; Light emitting diode; Toluidine blue O; Candida spp.; Photosensitizers
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In vitro photodynamic inactivation of Candida spp. growth and adhesion to buccal epithelial cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 94, Issue 1, 9 January 2009, Pages 65–70
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us