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Evaluation of culture conditions of locally isolated Dunaliella salina strain EgeMacc-024

Paper ID Volume ID Publish Year Pages File Format Full-Text
3094 150 2014 6 PDF Available
Title
Evaluation of culture conditions of locally isolated Dunaliella salina strain EgeMacc-024
Abstract

•Response surface methodology was used to optimize physical conditions of Dunaliella salina.•Light intensities, temperatures and agitation rates were investigated.•The developed mathematical model was validated and optimized results are reliable.

The aim of this study was to optimize physical parameters such as light intensity, temperature and agitation rate which might affect the cultivation of Dunaliella salina strain EgeMacc-024. For this purpose, a set of experiments were designed by central composite design using response surface methodology to statistically evaluate the findings. The optimal growth conditions were found at 22.09 °C under the light intensity of 55.75 μmol photons m−2 s−1 at the agitation rate of 148.73 rpm with the chlorophyll-a concentration of 6.47 mg/L, whereas the protein amount was 14.87 μg/100 μL at 27.97 °C under the light intensity of 57.5 μmol photons m−2 s−1 with the agitation rate of 166.08 rpm for D. salina. The maximum specific growth rate of 0.22 day−1, which corresponded to the doubling time of 3.15 day, was obtained for D. salina under determined conditions.

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Keywords
Agitation; Batch processing; Microalgae; Optimization; Dunaliella salina; Light intensity
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Evaluation of culture conditions of locally isolated Dunaliella salina strain EgeMacc-024
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 92, 15 November 2014, Pages 22–27
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us