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Photo-activated 5-hydroxyindole-3-acetic acid induces apoptosis of prostate and bladder cancer cells

Paper ID Volume ID Publish Year Pages File Format Full-Text
30953 44520 2011 7 PDF Available
Title
Photo-activated 5-hydroxyindole-3-acetic acid induces apoptosis of prostate and bladder cancer cells
Abstract

5-Hydroxyindole-3-acetic acid (5-HIAA), an indole derivative, is the main metabolite of serotonin in the human body. We determined whether or not ultraviolet B (UVB)-activated 5-HIAA (5-HIAAUVB) affects the viability of human prostate (LnCaP and PC-3) and bladder cancer cells (TCCSUP). While 5-HIAA alone had no cytotoxic effect at <1 mM, 5-HIAAUVB induced LnCaP, PC-3, and TCCSUP cell death in a time- and dose-dependent manner. Cell cycle analysis showed that 5-HIAAUVB markedly increased the sub-G0/G1 phase and resulted in cell cycle disruption. To elucidate the death mechanism by 5-HIAAUVB, we examined the signal transduction pathways related to apoptosis using Western blot analysis. 5-HIAAUVB led to phosphorylation of stress-activated signaling proteins, such as c-Jun N-terminal kinase (JNK) and/or p38 mitogen-activated protein kinase (MAPK). Furthermore, 5-HIAAUVB activated caspase-8, -9, and -3 and cleaved poly(ADP-ribose) polymerase (PARP), which are indicators of apoptosis. From these findings, the present study demonstrated that 5-HIAAUVB induces apoptotic cell death of prostate and bladder cancer cells via stress-mediated signaling and apoptotic pathways. Therefore, we suggest that 5-HIAA might be used as a new photosensitizer for photodynamic cancer therapy.

Keywords
5-Hydroxyindole-3-acetic acid; Ultraviolet B; Prostate cancer; Bladder cancer; Apoptosis
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Photo-activated 5-hydroxyindole-3-acetic acid induces apoptosis of prostate and bladder cancer cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 103, Issue 1, 4 April 2011, Pages 50–56
Authors
, , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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