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Extracellular β-class carbonic anhydrase of the alkaliphilic cyanobacterium Microcoleus chthonoplastes

Paper ID Volume ID Publish Year Pages File Format Full-Text
30956 44520 2011 9 PDF Available
Title
Extracellular β-class carbonic anhydrase of the alkaliphilic cyanobacterium Microcoleus chthonoplastes
Abstract

The gene for β-class carbonic anhydrase (CA), which was designated as cahB1, was cloned from the genomic library of the alkaliphilic cyanobacterium Microcoleus chthonoplastes. The product of the cahB1 gene was expressed in Escherichia coli. The protein revealed high specific activity of CA, which was inhibited with ethoxyzolamide. The maximum activity of the recombinant CA was detected at alkaline pH (∼9.0) and its minimum – at neutral pH (∼7.0). Western blotting analysis with the antibodies raised against the recombinant CahB1 protein revealed its localization in cell envelopes of M. chthonoplastes. Immunocytochemical localization of the CahB1 in cells confirmed its extracellular location. The newly characterized CahB1 of Microcoleus was similar in amino acid and nucleotide sequences to well known β-CAs of Synechococcus sp. PCC 7942 (IcfA) and Synechocystis sp. PCC 6803 (CcaA), although those CAs were attributed to the carboxysomal shells of cyanobacteria. Previously we have reported β-class CA which was associated with PS II of alkaliphilic cyanobacteria. Here we first report extracellular localization of β-class CA and provide a scheme for its possible involvement in the maintenance of a balance between external sources of inorganic carbon and photosynthesis in extreme environments of soda lakes.

► We cloned the gene for β-carbonic anhydrase (cahB1) from Microcoleus chthonoplastes. ► We demonstrated the extracellular localization of the CahB1 protein. ► The maximum activity of the CahB1 was detected at alkaline pH near 9.0. ► The biological role of the CahB1 in extreme environments of soda lakes is discussed.

Keywords
Microcoleus chthonoplastes; Cyanobacteria; Carbonic anhydrase; CCM; Soda lakes
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Extracellular β-class carbonic anhydrase of the alkaliphilic cyanobacterium Microcoleus chthonoplastes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 103, Issue 1, 4 April 2011, Pages 78–86
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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