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Improved detection of toxic chemicals by Photobacterium phosphoreum using modified Boss medium

Paper ID Volume ID Publish Year Pages File Format Full-Text
30960 44522 2010 6 PDF Available
Title
Improved detection of toxic chemicals by Photobacterium phosphoreum using modified Boss medium
Abstract

A bioluminescent bacterium Photobacterium phosphoreum has been used widely as an indicator of pollutants where the presence of toxic chemicals decreases light output. The optimum conditions for the growth and bioluminescence of P. phosphoreum KCTC 2852 were investigated using modified Boss medium and two environmental conditions (pH and temperature). Optimized conditions that supported growth of P. phosphoreum with high bioluminescence intensities were: NaCl (20 g/L), glycerol (2.5 g/L), peptone (1.0 g/L), and yeast extract (1.0 g/L). Growth and bioluminescence gradually increased as pH increased reaching a maximum at pH 7.0. The maximum temperature for growth and bioluminescence was 20 °C. Based on these optimum conditions for bioluminescence, a continuous culture reactor of P. phosphoreum was operated. During the continuous operation, the optimized medium maintained bioluminescence with a high relative light unit (RLU) around 13,600 for more than 120 h. When zinc (0.5 mg/L) was added to the reactor, an EC50 was observed in 15 min. The detection limit was improved by using the modified Boss medium since the modified Boss medium contained less organic matter which minimizes the complexation and precipitation of heavy metals compared to other enrichment media with high levels of organic matter.

Keywords
Photobacterium phosphoreum; Bioluminescence; Toxicity; Boss medium; Continuous culture
First Page Preview
Improved detection of toxic chemicals by Photobacterium phosphoreum using modified Boss medium
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 101, Issue 1, 5 October 2010, Pages 16–21
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering