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Detailed spectroscopic investigations to reveal the nature of interaction of anionic porphyrin with calf thymus DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
30969 44522 2010 8 PDF Available
Title
Detailed spectroscopic investigations to reveal the nature of interaction of anionic porphyrin with calf thymus DNA
Abstract

The interaction between anionic form of meso-tetrakis(4-carboxyphenyl) porphyrin (TCPP) and calf thymus deoxyribonucleic acid (CT DNA) is investigated by measuring UV–vis absorption, steady-state fluorescence, steady-state fluorescence anisotropy, time-resolved fluorescence, resonance light scattering (RLS), FT-IR and circular dichroism (CD) spectra along with the help of atomic force microscopy (AFM) under Tris–Borate–EDTA (TBE) buffer solution of pH 8.3. The static mode of fluorescence quenching of porphyrin by calf thymus deoxyribonucleic acid indicates the formation of a ground-state complex. The formation of ground-state complex is a spontaneous molecular interaction procedure in which outside groove binding through hydrogen bond or van der Waals force plays a major role. For biomedical application this investigation is very important as here TCPP, i.e. the anionic porphyrin does not bring any changes in the original structure of the CT DNA to selectively cleaving the nucleic acid to destroy the cancer or tumor cells whereas cationic porphyrin makes change in the protein structure significantly during the same process.

Keywords
Porphyrin; Deoxyribonucleic acid; Fluorescence anisotropy; Circular dichroism; Hydrogen bond; Static quenching
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Detailed spectroscopic investigations to reveal the nature of interaction of anionic porphyrin with calf thymus DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 101, Issue 1, 5 October 2010, Pages 89–96
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us