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Photoinactivation of bacteria in wastewater by porphyrins: Bacterial β-galactosidase activity and leucine-uptake as methods to monitor the process

Paper ID Volume ID Publish Year Pages File Format Full-Text
30977 44523 2007 7 PDF Available
Title
Photoinactivation of bacteria in wastewater by porphyrins: Bacterial β-galactosidase activity and leucine-uptake as methods to monitor the process
Abstract

The increasing interest in photodynamic antimicrobial chemotherapy as a promising technology for wastewater treatment demands more efficient photosensitizers and faster methods for the monitorization of the photoinactivation process. Two sets of neutral and cationic porphyrins were used as photosensitizers for the photoinactivation of sewage bacteria. Two of the cationic porphyrins revealed to be highly active, even against Gram-negative bacteria, inactivating ca. 94–99.8% of these at 5 μM and after 270 min of irradiation with white light (9 mW cm−2). In addition, two expeditious methods for the evaluation of the bacteria photoinactivation were developed. These methods are based on β-galactosidase activity and on leucine incorporation and give an excellent relation with faecal coliform counts. The evaluation of these two descriptors of bacterial activity, directly related with bacterial abundance, is an easy and fast way to monitor the micro-organisms during the photoinactivation process.

Keywords
Porphyrins; PACT; PDT; Photoinactivation; Faecal coliforms; Wastewater
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Photoinactivation of bacteria in wastewater by porphyrins: Bacterial β-galactosidase activity and leucine-uptake as methods to monitor the process
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 88, Issues 2–3, 25 September 2007, Pages 112–118
Authors
, , , , , , , , , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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Any Questions? feel free to contact us