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Bioluminescent monitoring of detoxification processes: Activity of humic substances in quinone solutions

Paper ID Volume ID Publish Year Pages File Format Full-Text
30980 44523 2007 6 PDF Available
Title
Bioluminescent monitoring of detoxification processes: Activity of humic substances in quinone solutions
Abstract

This study deals with application of bioluminescent assay systems to evaluate the detoxifying effect of humic substances (HS) on the solutions of organic oxidizers – quinones. A series of homologous quinones with different redox characteristics: 1,4-benzoquinone, tetrafluoro-1,4-benzoquinone, methyl-1,4-benzoquinone, tetramethyl-1,4-benzoquinone, and 1,4-naphtoquinone, was used. Bioluminescent bacteria Photobacterium phosphoreum, and NADH:FMN-oxidoreductase-luciferase enzyme system isolated from these bacteria were used as assay systems. The toxicity was compared in the presence and in the absence of HS. Variation of complexity of bioassays (in vivo or in vitro) combined with spectrometric and microscopic methods, provides insight into the process of detoxification in quinone solutions. Two ways of HS effect were studied: the reduction activity of HS and intensification of self-protection of bacterial cells on HS addition.

Keywords
Bioluminescent assay; Detoxification; Humic substances; Quinones
First Page Preview
Bioluminescent monitoring of detoxification processes: Activity of humic substances in quinone solutions
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 88, Issues 2–3, 25 September 2007, Pages 131–136
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering