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Time-dependent responses of wounded human skin fibroblasts following phototherapy

Paper ID Volume ID Publish Year Pages File Format Full-Text
30982 44523 2007 9 PDF Available
Title
Time-dependent responses of wounded human skin fibroblasts following phototherapy
Abstract

Background and objectiveThe penetration and distribution of laser light in target tissue is dependent on the wavelength of the light. One problem with most of the published data on laser irradiation is that most studies do not record the duration between the exposure and the evaluation. This study aimed to establish if the dose, wavelength or duration of effect (1 h or 24 h) influences the biological responses of irradiated fibroblasts.Materials and methodsThe study established cellular responses of normal and wounded human skin fibroblasts to helium–neon (632.8 nm), diode (830 nm) and Nd:YAG (1064 nm) laser irradiation using one exposure of 5 J/cm2 or 16 J/cm2 on day 1 and again on day 4. Cellular responses to laser irradiation were evaluated by measuring changes in cell viability (ATP viability and caspase 3/7 activity) and cell proliferation (ALP enzyme activity and bFGF expression), 1 h and 24 h post irradiation.ResultsWounded cells exposed to 5 J/cm2 using 632.8 nm showed an increase in ATP viability after 1 h, a decrease in caspase 3/7 activity after 24 h and an increase in cell proliferation after 24 h. The results suggest that changes in parameters such as ATP viability should be observed directly after laser irradiation (1 h) whereas other parameters such as caspase 3/7 activity, bFGF expression and ALP enzyme activity should be measured at least 24 h after the final exposure.ConclusionThis study confirms that the duration of effect should be included as one of the main laser parameters when reporting on the effects of laser irradiation. It is important to establish time-dependent responses as the results may provide an understanding of the cellular responses following laser irradiation.

Keywords
Central scratch; Duration; Diode; Helium–neon; Nd:YAG; Photobiomodulation; Wound
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 88, Issues 2–3, 25 September 2007, Pages 147–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us