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Probing oxidative stress in single erythrocytes with Raman Tweezers

Paper ID Volume ID Publish Year Pages File Format Full-Text
31007 44528 2010 4 PDF Available
Title
Probing oxidative stress in single erythrocytes with Raman Tweezers
Abstract

Raman Tweezers have been successfully applied to characterize chemically-induced oxidative stress on optically-trapped live, single erythrocytes. There is significant enhancement in Raman peak intensities corresponding to SS and C–S stretching modes that are induced by oxidative stress. This is consistent with the formation of mixed disulphides between protein SH groups and low-molecular-mass thiols such as glutathione during oxidative damage to cells. Enhancement in glutathione level as a protective response against oxidative stress has been observed. Principal component analysis of the data yields good discrimination between spectra of normal and stress-induced red blood cells.

Keywords
Raman Tweezers; Oxidative stress; Optical trap; Raman spectroscopy; Optical tweezers; Red blood cells
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 100, Issue 3, 2 September 2010, Pages 113–116
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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