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β-Carboline alkaloids bind DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
31046 44533 2010 8 PDF Available
Title
β-Carboline alkaloids bind DNA
Abstract

β-Carboline alkaloids present in Peganum harmala (harmal) have recently drawn attention due to their antitumor activities. The mechanistic studies indicate that beta-carboline derivatives inhibit DNA topoisomerases and interfere with DNA synthesis. They interact with DNA via both groove binding and intercalative modes and cause major DNA structural changes.The aim of this study was to examine the interactions of five β-carboline alkaloids (harmine, harmane, harmaline, harmalol and tryptoline) with calf-thymus DNA in aqueous solution at physiological conditions, using constant DNA concentration (6.25 mM) and various alkaloids/polynucleotide (phosphate) ratios of 1/240, 1/160, 1/80, 1/40, 1/20, 1/10, 1/5, 1/2 and 1/1. Fourier transform infrared (FTIR) and UV–visible spectroscopic methods were used to determine the ligand binding modes, the binding constants, and the stability of alkaloids–DNA complexes in aqueous solution.Spectroscopic evidence showed major binding of alkaloids to DNA with overall binding constants of Kharmine − DNA = 3.44 × 107 M−1, Kharmane − DNA = 1.63 × 105 M−1, Kharmaline − DNA = 3.82 × 105 M−1, Kharmalol − DNA = 6.43 × 105 M−1 and Ktryptoline − DNA = 1.11 × 105 M−1. The affinity of alkaloids–DNA binding is in the order of harmine > harmalol > harmaline > harmane > tryptoline. No biopolymer secondary structural changes were observed upon alkaloid interaction and DNA remains in the B-family structure in these complexes.

Keywords
DNA; β-Carboline alkaloids; FTIR; UV–visible spectroscopy
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β-Carboline alkaloids bind DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 100, Issue 2, 2 August 2010, Pages 84–91
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us