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Influence of photodynamic effect on biological activity of PBR–PP complexes

Paper ID Volume ID Publish Year Pages File Format Full-Text
31090 44539 2010 8 PDF Available
Title
Influence of photodynamic effect on biological activity of PBR–PP complexes
Abstract

The aim of this study was to examine the influence of photodynamic effect on biological activity of PBR–PP complexes. These measurements were performed in pH dependent environment. Constant concentration of solubilized receptor was titrated with increasing concentration of porphyrins (PPIX, Hp, PP(Arg)2, Hp(Arg)2, PP(Gly)2, PP(Ala)2, PP(Ser)2, PP(Phe)2) and binding constants were calculated. PBP–PP mixtures were illuminated with 3 J, 5 J or 10 J of blue light and changes in protein fluorescence was recorded. Experimental data were fitted to weak and strong binding models. As a result for all derivatives weak binding model was the best fitted. The strongest binding showed PPIX in pH 7.4 and with pH drop binding constants showed greater values for all examined derivatives. Out of amino acid derivatives the strongest binding was noticed for PP(Gly)2 and PP(Phe)2 and for the last one pH influence was not observed.

Keywords
PBR; Photodynamic effect; Binding constant
First Page Preview
Influence of photodynamic effect on biological activity of PBR–PP complexes
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 98, Issue 2, 12 February 2010, Pages 159–166
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering