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Disaggregation of amphotericin B by sodium deoxycholate micellar aggregates

Paper ID Volume ID Publish Year Pages File Format Full-Text
31121 44549 2008 5 PDF Available
Title
Disaggregation of amphotericin B by sodium deoxycholate micellar aggregates
Abstract

Amphotericin B (AmB) is present as aggregates in aqueous media. Sodium deoxycholate (NaDC) is known to cause disaggregation of AmB and to form monomers, as is observed by the increase of absorbance at 414 nm. By following the fluorescence intensity at 472 nm (λex 335 nm), it was found that with increasing NaDC concentration there is a progressive disaggregation of AmB leading to increase in both monomeric and dimeric forms. Although the presence of dimeric AmB form in NaDC micellar media is known, observation of the growth of the dimeric species is novel. Studies in fluorescence lifetime and steady state fluorescence anisotropy also support this conclusion. As NaDC (at ca. 10 mM) is the preferred choice of medium for AmB delivery and AmB aggregation has been implicated for its toxicity, the observation of dimeric AmB at these NaDC concentrations assumes significance.

Keywords
Amphotericin B; Sodium deoxycholate; Fluorescence; Absorbance; Disaggregation
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Disaggregation of amphotericin B by sodium deoxycholate micellar aggregates
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 93, Issue 2, 13 November 2008, Pages 66–70
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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