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Delayed fluorescence as a measure of nutrient limitation in Dunaliella tertiolecta

Paper ID Volume ID Publish Year Pages File Format Full-Text
31130 44552 2008 6 PDF Available
Title
Delayed fluorescence as a measure of nutrient limitation in Dunaliella tertiolecta
Abstract

The applicability of the delayed fluorescence (DF) for the purpose of distinguishing the cells growing in different nutrient conditions was researched on the marine unicellular algae Dunaliella tertiolecta Butcher (Chlorophyta). The DF intensity (DFI), as a measure of living algal biomass, was compared with other biomass measures – the cell concentration, chlorophyll a and fluorescence. The photosynthetic activity index (PhAI), a non-dimensional physiological index of photosynthesis calculated from a combination of DFI and F0 was introduced. The nitrogen deprivation was indicated by more than 50% drop of PhAI. DF decay kinetics was measured with two different illuminations (<600 nm and >650 nm). The measured curves were divided and the resulting peak utilized for the differentiation among nutrient conditions. DF decay kinetics of D. tertiolecta differed among the cells growing in various nutrient conditions, indicating changes in the photosynthesis physiology.

Keywords
Nutrient limitation; Nitrogen; Phosphorus; Delayed fluorescence; Fluorescence; Chlorophyll a; Dunaliella tertiolecta
First Page Preview
Delayed fluorescence as a measure of nutrient limitation in Dunaliella tertiolecta
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 92, Issue 1, 24 July 2008, Pages 13–18
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering