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Effect of phosphatidylglycerol depletion on the surface electric properties and the fluorescence emission of thylakoid membranes

Paper ID Volume ID Publish Year Pages File Format Full-Text
31146 44554 2008 7 PDF Available
Title
Effect of phosphatidylglycerol depletion on the surface electric properties and the fluorescence emission of thylakoid membranes
Abstract

To explore the possible effect of phosphatidylglycerol (PG) on the surface electric properties and chlorophyll fluorescence characteristics we used electric light scattering technique and 77 K chlorophyll fluorescence of thylakoid membranes from a cyanobacterium, Synechocystis PCC6803 (wild type) and its pgsA mutant defective in PG synthesis. We found a strong decrease in the permanent and induced electric dipole moments of the mutant thylakoids, following long-term PG depletion parallel with a decrease of the emission peak from PSI and an increase of the emission peak from PSII. Partial recovery of the electric state of thylakoid membranes was observed at re-addition of PG to the mutant cells depleted of PG for 21days. This change in the electric dipole moments is probably due to a decrease in PG content and progressive structural alterations in the macroorganization of the photosynthetic complexes induced by PG deprivation.Our results suggest that the depletion of a lipid, which carries a negative charge, despite its small contribution to the overall lipid content, significantly perturbs the surface charge of the membranes. These changes are related with the chlorophyll fluorescence emission ratios of two photosystems and may partly explain our earlier results concerning the PG requirement for the function and assembly of photosystems I and II reaction centers.

Keywords
Phosphatidylglycerol; Synechocystis PCC6803; pgsA mutant; Thylakoid membranes; Electric light scattering; Chlorophyll fluorescence
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Effect of phosphatidylglycerol depletion on the surface electric properties and the fluorescence emission of thylakoid membranes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 91, Issue 1, 25 April 2008, Pages 51–57
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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