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Algorithm for analysis of OJDIP fluorescence induction curves in terms of photo- and electrochemical events in photosystems of plant cells:: Derivation and application

Paper ID Volume ID Publish Year Pages File Format Full-Text
31147 44554 2008 8 PDF Available
Title
Algorithm for analysis of OJDIP fluorescence induction curves in terms of photo- and electrochemical events in photosystems of plant cells:: Derivation and application
Abstract

The algorithm for simulation of the OJDIP fluorescence induction curve in chloroplasts under variable conditions is presented. It is derived from analyzes of chlorophyll a fluorescence kinetics upon excitation with single- (STF), twin- (TTF) and repetitive STF excitations, and from the rate equations that describe the sequence of transfer steps associated with the reduction of the primary quinone acceptor QA and the release of photochemical fluorescence quenching of photosystem II (PSII) in multi-turnover excitation (MTF). The fluorescence induction algorithm (FIA) considers a photochemical O–J–D, a photo-electrochemical J–I and an I–P component (phase) which probably is associated with a photo-electric interaction between PSI and PSII. The photochemical phase incorporates the kinetics associated with the double reduction of the acceptor pair [PheQA] in QB–nonreducing reaction centers (RCs) and the associated doubling of the variable fluorescence, in agreement with the three-state trapping model (TSTM) of PSII. Application of and results with the algorithm are illustrated for MTF-induced OJDIP curves, measured in dark-adapted, in STF pre-excited and in DCMU inhibited thylakoids.

Keywords
Fluorescence induction algorithm; Energy trapping; Photosystem II; Model
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Algorithm for analysis of OJDIP fluorescence induction curves in terms of photo- and electrochemical events in photosystems of plant cells:: Derivation and application
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 91, Issue 1, 25 April 2008, Pages 58–65
Authors
,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us