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Spectroscopic probing of ortho-nitrophenol localization in phospholipid bilayers

Paper ID Volume ID Publish Year Pages File Format Full-Text
31157 44555 2007 7 PDF Available
Title
Spectroscopic probing of ortho-nitrophenol localization in phospholipid bilayers
Abstract

In the present study we estimated the localization of ortho-nitrophenol (ONP) within model membranes through its efficiency to quench and to modify the anisotropy of DPH and TMA-DPH fluorescence. These fluorescent probes are known to sense the hydrocarbon core and the polar head group region of membranes, respectively. TMA-DPH fluorescence in MLVs was more efficiently quenched than DPH (Kq,TMA-DPH = 2.36 and Kq,DPH = 1.07 mM ns−1). Moreover, these results demonstrated the interfacial localization of ONP and may contribute to understand membrane-mediated mechanisms of ONP-induced toxicity and the behavior of ONP as a product of several enzymatic reactions occurring in the presence of lipid–water interfaces.

Keywords
Ortho-nitrophenol localization; Phospholipid vesicle; DPH, TMA-DPH; Fluorescence quenching
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Spectroscopic probing of ortho-nitrophenol localization in phospholipid bilayers
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 89, Issue 1, 12 November 2007, Pages 56–62
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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