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Ultrastructural and autoradiographical analysis show a faster skin repair in He–Ne laser-treated wounds

Paper ID Volume ID Publish Year Pages File Format Full-Text
31159 44560 2007 10 PDF Available
Title
Ultrastructural and autoradiographical analysis show a faster skin repair in He–Ne laser-treated wounds
Abstract

There are evidences that low-intensity red laser radiation is capable to accelerate wound healing. Nowadays, this therapy has been gradually introduced in clinical practice although mechanisms underlying laser effects are poorly understood. To better understand the photobiological effects of laser radiation, this study investigated by electron microscopy, immunohistochemistry and autoradiography the morphological and functional features of irradiated and none irradiated injured mice skin. Full-thickness skin lesions were created on the back of mice and irradiated on days 1, 5, 8, 12, and 15 post-wounding with a He–Ne laser (λ = 632.8 nm), dose 1 J/cm2, exposure time 3 min. Non-irradiated lesions were used as a control. The mice were inoculated with 3H-proline and sacrificed one hour after on the 8th, 15th and 22nd days to histological and radioautographical analysis. The irradiated-lesions showed a faster reepithelization compared with control lesions. The irradiated dermis contained a higher number of activated fibroblasts compared to control group and, most of them showed several cytoplasmic collagen-containing phagosomes. In irradiated-lesions, smooth muscle α-actin positive cells predominated, which correspond to a higher number of myofibroblasts observed in the electron microscope. Moreover, laser radiation reduced the local inflammation and appears to influence the organization of collagen fibrils in the repairing areas. Quantitative autoradiography showed that the incorporation of 3H-proline was significantly higher in irradiated-dermis on the 15th day post-wounding (p < 0.05). These results suggest that laser radiation may accelerate cutaneous wound healing in a murine model.

Keywords
633 nm wavelength; Low-intensity laser therapy; Mice; Wound healing
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Ultrastructural and autoradiographical analysis show a faster skin repair in He–Ne laser-treated wounds
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 86, Issue 2, 1 February 2007, Pages 87–96
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us