fulltext.study @t Gmail

Bio-ethanol production through simultaneous saccharification and fermentation using an encapsulated reconstituted cell-free enzyme system

Paper ID Volume ID Publish Year Pages File Format Full-Text
3118 151 2014 10 PDF Available
Title
Bio-ethanol production through simultaneous saccharification and fermentation using an encapsulated reconstituted cell-free enzyme system
Abstract

•An encapsulated reconstituted cell-free enzyme system was developed.•The system effectively produced bio-ethanol at elevated temperature through SSF.•The system was successfully evaluated for bioethanol production till 15 repeated batches.•The kinetics profile of SSF process for this system was studied.•It offered several advantages and was superior compared to previously reported SSF systems.

An encapsulated reconstituted cell-free enzyme system was developed through liquid-droplet forming method by using endogenous glycolytic and fermentation enzymes from yeast cells and exogenously added saccharification enzymes, cofactors, and ATPase. It was evaluated for bio-ethanol production through simultaneous saccharification and fermentation (SSF) at various temperatures, pH, and cell-free enzyme and substrate concentrations. Using 1% starch as substrate, encapsulated system illustrated maximum efficiency at 45 °C and pH 7.0. SSF with encapsulated and bare reconstituted cell-free enzyme systems produced 3.47 g/L and 2.98 g/L bio-ethanol corresponding to 62% and 53% of maximum theoretical yield, respectively. It was explicable that encapsulated system provided better substrate utilization and product formation at elevated temperatures than bare system. Kinetic profile of SSF process of both systems was affected differently by variations in pH, temperature, and substrate and cell-free enzyme concentrations. Under appropriate conditions, system retained 90%, 64%, and 40% of initial enzyme concentration and produced 3.21, 2.24, and 0.83 g/L bio-ethanol after 5, 10, and 15 consecutive batches, respectively. The current system offered several advantages and was superior compared to previously reported SSF systems. This system can effectively overcome the major barriers associated with successful development of SSF processes for bio-ethanol production on an industrial scale.

Keywords
Encapsulation; Reconstituted cell-free system; Ethanol; Enzyme technology; Yeast; Batch processing
First Page Preview
Bio-ethanol production through simultaneous saccharification and fermentation using an encapsulated reconstituted cell-free enzyme system
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 91, 15 October 2014, Pages 110–119
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us