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Hydroxyapatite reinforced collagen scaffolds with improved architecture and mechanical properties ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
312 25 2015 10 PDF Available
Title
Hydroxyapatite reinforced collagen scaffolds with improved architecture and mechanical properties ☆
Abstract

Hydroxyapatite (HA) reinforced collagen scaffolds have shown promise for synthetic bone graft substitutes and tissue engineering scaffolds. Freeze-dried HA–collagen scaffolds are readily fabricated and have exhibited osteogenicity in vivo, but are limited by an inherent scaffold architecture that results in a relatively small pore size and weak mechanical properties. In order to overcome these limitations, HA–collagen scaffolds were prepared by compression molding HA reinforcements and paraffin microspheres within a suspension of concentrated collagen fibrils (∼180 mg/mL), cross-linking the collagen matrix, and leaching the paraffin porogen. HA–collagen scaffolds exhibited an architecture with high porosity (85–90%), interconnected pores ∼300–400 μm in size, and struts ∼3–100 μm in thickness containing 0–80 vol% HA whisker or powder reinforcements. HA reinforcement enabled a compressive modulus of up to ∼1 MPa, which was an order of magnitude greater than unreinforced collagen scaffolds. The compressive modulus was also at least one order of magnitude greater than comparable freeze-dried HA–collagen scaffolds and two orders of magnitude greater than absorbable collagen sponges used clinically. Moreover, scaffolds reinforced with up to 60 vol% HA exhibited fully recoverable elastic deformation upon loading to 50% compressive strain for at least 100,000 cycles. Thus, the scaffold mechanical properties were well-suited for surgical handling, fixation, and bearing osteogenic loads during bone regeneration. The scaffold architecture, permeability, and composition were shown to be conducive to the infiltration and differentiation of adipose-derive stromal cells in vitro. Acellular scaffolds were demonstrated to induce angiogenesis and osteogenesis after subcutaneous ectopic implantation by recruiting endogenous cell populations, suggesting that the scaffolds were osteoinductive.

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Keywords
Bioactivity; Collagen; Hydroxyapatite; Osteoinduction; Scaffold
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Hydroxyapatite reinforced collagen scaffolds with improved architecture and mechanical properties ☆
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 17, 15 April 2015, Pages 16–25
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us