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Utilization of nano-gold tracing technique: Study the adsorption and transmission of laccase in mediator-involved enzymatic degradation of lignin during solid-state fermentation

Paper ID Volume ID Publish Year Pages File Format Full-Text
3122 151 2014 8 PDF Available
Title
Utilization of nano-gold tracing technique: Study the adsorption and transmission of laccase in mediator-involved enzymatic degradation of lignin during solid-state fermentation
Abstract

•Nano-gold tracing technique was utilized to study the adsorption and transmission.•Mediator-involved enzymatic degradation of lignin was studied.•Effects of mediators on the degradation of lignin: veratryl alcohol > ABTS > Mn2+.•A higher lignin degradation ratio was found during solid-state fermentation.

The degradation of lignin waste by ligninolytic enzymes and the effect of mediators on lignin degradation were studied. Moreover, nano-gold tracing technique was innovatively used to study the adsorption and transmission mechanism of ligninolytic enzymes in the process of SSF. The results showed that the effects of three common mediators on the degradation of lignin were in descending order: veratryl alcohol > ABTS > Mn2+, and the optimum dosages were: Mn2+ (20 μM g−1), ABTS (0.2 μM g−1), veratryl alcohol (4 μM g−1). A significantly high degradation ratio of lignin (31.8%) was achieved at the optimum dosages of ligninolytic enzymes and mediators. In addition, laccase, as a typical ligninolytic enzyme, was selected to conjugate to nano-gold for the study of adsorption and transmission mechanism. An obvious decrease of nano-gold-laccase conjugates was observed after 10 days of enzymatic hydrolysis and there were few laccase residues on the surface of straw fibers after 30 days of enzymatic hydrolysis. The present findings will advance the understanding of mediator-involved enzymatic degradation of lignin, as well as the adsorption and transmission mechanism of ligninolytic enzymes in lignin degradation, which could provide useful references for developing waste biotreatment technology.

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Keywords
Enzymes; Lignin; Mediators; Solid-state fermentation; Nano-gold tracing technique; Adsorption
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Utilization of nano-gold tracing technique: Study the adsorption and transmission of laccase in mediator-involved enzymatic degradation of lignin during solid-state fermentation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 91, 15 October 2014, Pages 149–156
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us