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Effect of different salts and detergents on luciferin–luciferase luminescence of the enchytraeid Fridericia heliota

Paper ID Volume ID Publish Year Pages File Format Full-Text
31223 44570 2006 6 PDF Available
Title
Effect of different salts and detergents on luciferin–luciferase luminescence of the enchytraeid Fridericia heliota
Abstract

The study addresses the effect produced by different inorganic salts and detergents (SDS, Triton X-100, the Tween series) on the ATP-dependent bioluminescent reaction catalyzed by the luciferase of the new earthworm species Fridericia heliota (Annelida: Clitellata: Oligochaeta: Enchytraeidae). It has been shown that the effect of divalent metal salts on luminescence is determined by the action of cations. Three of them – Mg2+, Mn2+ and Ca2+ – can stimulate luciferase activity at concentrations varying within a wide range, and Mn2+ can act as a 100%-effective substitute for Mg2+ in F. heliota   luminescence reaction in vitro. The inhibitory effect of monovalent metal salts on luminescence is largely determined by the action of the anion part of the molecule. The effectiveness of the inhibitory effect of anions increases in the following order: Cl-

Keywords
Bioluminescence; Earthworms; Luciferin–luciferase systems; ATP; Ions; Triton X-100
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Effect of different salts and detergents on luciferin–luciferase luminescence of the enchytraeid Fridericia heliota
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Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology B: Biology - Volume 83, Issue 2, 1 May 2006, Pages 123–128
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us