fulltext.study @t Gmail

ECHO probes: Fluorescence emission control for nucleic acid imaging

Paper ID Volume ID Publish Year Pages File Format Full-Text
31332 44742 2012 12 PDF Available
Title
ECHO probes: Fluorescence emission control for nucleic acid imaging
Abstract

The understanding of the regulation of the mechanisms via which genomic information dictates cellular behaviors has become a great challenge of the postgenomic era. Tools that allow sensitive, quantitative, and real-time detection of specific transcripts, as well as the study of spatiotemporal gene regulation in living cells, are being developed. In this article, we review recent advances in nucleic acid detection using exciton-controlled hybridization-sensitive fluorescent oligonucleotide (ECHO) probe technologies. In ECHO probes, a hybridization-dependent fluorescent nucleotide regulated by the H-aggregation of thiazole orange organic dyes (D514) is incorporated into specific sequence contexts and serves as fluorescent detection readout for target nucleic acids. Multicolor detection and auxiliary functional modules have been built into ECHO probes to accommodate a broad range of biological applications.

► Excitonic interaction suppresses fluorescence emission of thiazole orange homodimers. ► Suppression release in recognition of target nucleic acids robustly turns on multicolor fluorescence. ► Versatile functional modules allow a broad range of applications.

Keywords
Excitonic interaction; Functional oligodeoxynucleotides; Live-cell RNA imaging; Single nucleotide polymorphism detection
First Page Preview
ECHO probes: Fluorescence emission control for nucleic acid imaging
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Journal of Photochemistry and Photobiology C: Photochemistry Reviews - Volume 13, Issue 2, June 2012, Pages 112–123
Authors
, ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us