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Directed evolution of Bacillus licheniformis lipase for improvement of thermostability

Paper ID Volume ID Publish Year Pages File Format Full-Text
3135 151 2014 7 PDF Available
Title
Directed evolution of Bacillus licheniformis lipase for improvement of thermostability
Abstract

•Thermostability of Bacillus licheniformis lipase improved by directed evolution.•The best mutant lipase (eP-231-51) was selected after two rounds of ep-PCR.•eP-231-51 showed 13.5-fold increase in percentage thermal stability.•The best mutant lipases were also resistant to hydrophobic organic solvents.•The role of mutations that led to thermostability have been postulated.

Lipases catalyze the hydrolysis of carboxylic acid esters and owing to their vast substrate specificity, they have many industrial applications. Due to the demand of thermostable lipases in industrial applications, we have enhanced the thermostability of lipase from Bacillus licheniformis RSP-09. The thermostable mutant lipases of Bacillus licheniformis RSP-09 were isolated following two rounds of directed evolution using error-prone PCR. The best mutant lipases obtained after first and second round of error-prone PCR were purified and characterized. The mutant lipases showed increased thermostability and retained catalytic function. The best mutant lipase (eP-231-51) showed 13.5-fold increase in percentage thermal stability (% remaining activity after incubation of purified enzyme at 60 °C for 1 h) than wild-type lipase. Also, this mutant lipase (ep-231-51) showed 30% improved catalytic efficiency compared with the wild-type which is due to significant decrease in Km and marginal increase in kcat. In addition, the thermostable mutant lipases have shown resistance to hydrophobic organic solvents. The role of mutations in the best mutant lipases of second round i.e. eP-231-51 (Asp72Gly, Asp61Gly, Tyr129His, and Thr101Pro) and eP-231-137 (Leu49Arg, Thr101Pro, Asp72Gly), that led to thermostability have been postulated after the comparison of molecular models of wild-type and mutated enzymes.

Keywords
Lipase; Biocatalysis; Enzymes; Protein; Directed evolution; Thermostability
First Page Preview
Directed evolution of Bacillus licheniformis lipase for improvement of thermostability
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 91, 15 October 2014, Pages 276–282
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering