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Biomimetic design of affinity peptide ligands for human IgG based on protein A-IgG complex

Paper ID Volume ID Publish Year Pages File Format Full-Text
3148 153 2014 11 PDF Available
Title
Biomimetic design of affinity peptide ligands for human IgG based on protein A-IgG complex
Abstract

•Biomimetic design strategy of affinity peptide ligands for hIgG was developed.•Affinity peptide library for hIgG was built based on the affinity model of protein A.•The library was screened by multi-level dockings, RMSD comparison and MD simulations.•Affinity and specificity of the top ligand were verified by hIgG chromatography.

Staphylococcal protein A (SpA) has been widely used as an affinity ligand for the purification of immunoglobulin G (IgG). Based on the affinity motif of SpA, we have herein developed a biomimetic design strategy for affinity peptide ligands of IgG. First, according to the distribution of the six hot spots of the SpA affinity motif determined previously, the number of residues that should be inserted into between the hot spots was determined. Cysteine was introduced as one of the middle inserted residues of the peptide for later immobilization. Then, amino acid location was performed to identify other amino acid residues for insertion, leading to the construction of a peptide library. The library was screened by using different molecular simulation protocols, resulting in the selection of 15 peptide candidates. Thereafter, molecular dynamics simulations were performed to validate the dynamics of the affinity interactions between the candidates and IgG, and 14 of them were found to keep high affinities. Finally, the affinity and specificity of the top one ligand FYWHCLDE were exemplified by protein chromatography and IgG purification. The results indicate that the design strategy was successful and the affinity peptide ligand for IgG is promising for application in antibody purifications.

Keywords
Protein; Affinity; Adsorption; Chromatography; Purification; Molecular simulation
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Biomimetic design of affinity peptide ligands for human IgG based on protein A-IgG complex
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 88, 15 July 2014, Pages 1–11
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
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