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Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes

Paper ID Volume ID Publish Year Pages File Format Full-Text
31481 44800 2016 10 PDF Available
Title
Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes
Abstract

•Tunable biofilm formation and dispersal achieved in Pseudomonas putida.•Tight expression of enzymes controlling cyclic diguanosine monophosphate levels.•Catalytic biofilms of Pseudomonas putida displaying a high dehalogenase activity.•Natural advantages of a hefty biocatalyst exploited in a novel, controllable setup.•First report on the manipulation of synthetic morphology in whole-cell biocatalysis.

Bacterial biofilms outperform planktonic counterparts in whole-cell biocatalysis. The transition between planktonic and biofilm lifestyles of the platform strain Pseudomonas putida KT2440 is ruled by a regulatory network controlling the levels of the trigger signal cyclic di-GMP (c-di-GMP). This circumstance was exploited for designing a genetic device that over-runs the synthesis or degradation of c-di-GMP – thus making P. putida to form biofilms at user׳s will. For this purpose, the transcription of either yedQ (diguanylate cyclase) or yhjH (c-di-GMP phoshodiesterase) from Escherichia coli was artificially placed under the tight control of a cyclohexanone-responsive expression system. The resulting strain was subsequently endowed with a synthetic operon and tested for 1-chlorobutane biodegradation. Upon addition of cyclohexanone to the culture medium, the thereby designed P. putida cells formed biofilms displaying high dehalogenase activity. These results show that the morphologies and physical forms of whole-cell biocatalysts can be genetically programmed while purposely designing their biochemical activity.

Keywords
Pseudomonas putida; Catalytic biofilm; Dehalogenase; Metabolic engineering; Biocatalysis; Synthetic morphology
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Genetic programming of catalytic Pseudomonas putida biofilms for boosting biodegradation of haloalkanes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 33, January 2016, Pages 109–118
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Any Questions? feel free to contact us