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Transcriptional feedback regulation of efflux protein expression for increased tolerance to and production of n-butanol

Paper ID Volume ID Publish Year Pages File Format Full-Text
31483 44800 2016 8 PDF Available
Title
Transcriptional feedback regulation of efflux protein expression for increased tolerance to and production of n-butanol
Abstract

•A genetic network that optimizes membrane protein expression is created in E. coli.•Controlling expression of a butanol-secreting pump gives higher n-butanol titers.•The system is tunable and generalizable to other toxic membrane proteins.

Microorganisms can be engineered to produce a variety of biofuels and commodity chemicals. The accumulation of these products, however, is often toxic to the cells and subsequently lowers production yields. Efflux pumps are a natural mechanism for alleviating toxicity through secretion of the product; unfortunately, pump overexpression also often inhibits growth. Tuning expression levels with inducible promoters is time-consuming and the reliance on small-molecule inducers is cost-prohibitive in industry. We design an expression regulation system utilizing a native Escherichia coli stress promoter, PgntK, to provide negative feedback to regulate transporter expression levels. We test the promoter in the context of the efflux pump AcrB and its butanol-secreting variant, AcrBv2. PgntK-driven AcrBv2 confers increased tolerance to n-butanol and increased titers of n-butanol in production. Furthermore, the system is responsive to stress from toxic overexpression of other membrane-associated proteins. Our results suggest a use for feedback regulation networks in membrane protein expression.

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Keywords
AcrB; Dynamic gene regulation; Membrane protein overexpression; N-butanol production
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Transcriptional feedback regulation of efflux protein expression for increased tolerance to and production of n-butanol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 33, January 2016, Pages 130–137
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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Online Support
Any Questions? feel free to contact us