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Microbial production of bi-functional molecules by diversification of the fatty acid pathway

Paper ID Volume ID Publish Year Pages File Format Full-Text
31489 44803 2016 12 PDF Available
Title
Microbial production of bi-functional molecules by diversification of the fatty acid pathway
Abstract

Fatty acids that are chemically functionalized at their ω-ends are rare in nature yet offer unique chemical and physical properties with wide ranging industrial applications as feedstocks for bio-based polymers, lubricants and surfactants. Two enzymatic determinants control this ω-group functionality, the availability of an appropriate acyl-CoA substrate for initiating fatty acid biosynthesis, and a fatty acid synthase (FAS) variant that can accommodate that substrate in the initial condensation reaction of the process. In Type II FAS, 3-ketoacyl-ACP synthase III (KASIII) catalyses this initial condensation reaction. We characterized KASIIIs from diverse bacterial sources, and identified variants with novel substrate specificities towards atypical acyl-CoA substrates, including 3-hydroxybutyryl-CoA. Using Alicyclobacillus acidocaldarius KASIII, we demonstrate the in vivo diversion of FAS to produce novel ω-1 hydroxy-branched fatty acids from glucose in two bioengineered microbial hosts. This study unveils the biocatalytic potential of KASIII for synthesizing diverse ω-functionalized fatty acids.

Keywords
KASIII, 3-Ketoacyl-ACP-Synthase III; HBFA, ω-1 Hydroxy Branched Fatty Acid; FAS, Fatty Acid Synthase; PHB, Polyhydroxybutyrate3-Ketoacyl-ACP Synthase III; Fatty acid synthesis; Bio-based chemicals; Omega-functionalized fatty acids; Substrate diversity; Mi
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Microbial production of bi-functional molecules by diversification of the fatty acid pathway
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 35, May 2016, Pages 9–20
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
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