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Coenzyme regeneration catalyzed by NADH oxidase from Lactococcus lactis

Paper ID Volume ID Publish Year Pages File Format Full-Text
3149 153 2014 7 PDF Available
Title
Coenzyme regeneration catalyzed by NADH oxidase from Lactococcus lactis
Abstract

•Partially purified NADH oxidase from Lactococcus lactis was kinetically characterized.•Only low oxygen concentration is required to achieve maximum enzyme activity.•Enzyme is sensitive to air/liquid interface.•Enzyme was successfully used for coenzyme regeneration during l-methionine oxidation.

Lactococcus lactis was aerobically grown in a bioreactor to produce NADH oxidase, an enzyme used for NAD+ regeneration. The enzyme was isolated and purified from the cells that were harvested at the end of exponential phase of growth. The influence of temperature, pH and oxygen on enzyme activity was investigated. The enzyme was kinetically characterized at different pH values and in different buffers. It was found that Michaelis constants for oxygen are very low (the highest was 4.5 μmol dm−3 at pH 8.0). NADH oxidase was tested as a regenerating enzyme in a model system of l-methionine oxidation catalyzed by l-phenylalanine dehydrogenase from Rhodococcus sp. When NADH oxidase from L. lactis was used for NAD+ regeneration, 100% l-methionine conversion was achieved, while without regeneration of NAD+ it was estimated to be about 28%. The operational stability of NADH oxidase was followed and it was found that enzyme activity decay occurs. The operational stability decay rate constant, kd, was estimated to be 8.0 × 10−5 min−1.

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Keywords
Lactococcus lactis; NADH oxidase; Coenzyme NAD+ regeneration
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Coenzyme regeneration catalyzed by NADH oxidase from Lactococcus lactis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 88, 15 July 2014, Pages 12–18
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us