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Characterizing MttA as a mitochondrial cis-aconitic acid transporter by metabolic engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
31498 44803 2016 10 PDF Available
Title
Characterizing MttA as a mitochondrial cis-aconitic acid transporter by metabolic engineering
Abstract

•MttA is a mitochondrial cis-aconitate transporter from A. terreus.•MttA increases itaconic acid production.•Metabolic engineering can be used to reveal the substrate characteristics of transport proteins in vivo.•MttA can be used to engineer cells for the secretion of aconitic acid.

The mitochondrial carrier protein MttA is involved in the biosynthesis of itaconic acid in Aspergillus terreus. In this paper, the transport specificity of MttA is analyzed making use of different metabolically engineered Aspergillus niger strains. Furthermore, the mitochondrial localization of this protein is confirmed using fluorescence microscopy. It was found that MttA preferentially transports cis-aconitic acid over citric acid and does not transport itaconic acid. The expression of MttA in selected A. niger strains results in secretion of aconitic acid. MttA can be used in further strain engineering strategies to transport cis-aconitic acid to the cytosol to produce itaconic acid or related metabolites. The microbial production of aconitic acid (9 g/L) is achieved in strains expressing this transport protein. Thus, metabolic engineering can be used for both the in vivo characterization of transport protein function like MttA and to make use of this protein by creating aconitic acid producing strains.

Keywords
Itaconic acid; Aspergillus terreus; Trans-aconitic acid; Mitochondrial carrier protein; Aspergillus niger; Doxycycline; Itaconate; Aconitate
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Characterizing MttA as a mitochondrial cis-aconitic acid transporter by metabolic engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 35, May 2016, Pages 95–104
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us