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Membrane engineering via trans unsaturated fatty acids production improves Escherichia coli robustness and production of biorenewables

Paper ID Volume ID Publish Year Pages File Format Full-Text
31499 44803 2016 9 PDF Available
Title
Membrane engineering via trans unsaturated fatty acids production improves Escherichia coli robustness and production of biorenewables
Abstract

•TUFA production significantly improves tolerance and production of carboxylic acids.•TUFA production significantly improves tolerance and production of styrene.•Tuning of the TUFA relative abundance impacts effectiveness.•TUFA production increases membrane rigidity, does not impact membrane integrity.•TUFA production improves thermotolerance, growth at pH 5.5 and butanol tolerance.

Constructing microbial biocatalysts that produce biorenewables at economically viable yields and titers is often hampered by product toxicity. For production of short chain fatty acids, membrane damage is considered the primary mechanism of toxicity, particularly in regards to membrane integrity. Previous engineering efforts in Escherichia coli to increase membrane integrity, with the goal of increasing fatty acid tolerance and production, have had mixed results. Herein, a novel approach was used to reconstruct the E. coli membrane by enabling production of a novel membrane component. Specifically, trans unsaturated fatty acids (TUFA) were produced and incorporated into the membrane of E. coli MG1655 by expression of cis-trans isomerase (Cti) from Pseudomonas aeruginosa. While the engineered strain was found to have no increase in membrane integrity, a significant decrease in membrane fluidity was observed, meaning that membrane polarization and rigidity were increased by TUFA incorporation. As a result, tolerance to exogenously added octanoic acid and production of octanoic acid were both increased relative to the wild-type strain. This membrane engineering strategy to improve octanoic acid tolerance was found to require fine-tuning of TUFA abundance. Besides improving tolerance and production of carboxylic acids, TUFA production also enabled increased tolerance in E. coli to other bio-products, e.g. alcohols, organic acids, aromatic compounds, a variety of adverse industrial conditions, e.g. low pH, high temperature, and also elevated styrene production, another versatile bio-chemical product. TUFA permitted enhanced growth due to alleviation of bio–product toxicity, demonstrating the general effectiveness of this membrane engineering strategy towards improving strain robustness.

Keywords
Trans unsaturated fatty acids (TUFA); Tolerance; Membrane fluidity; Membrane integrity; Carboxylic acids production
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Membrane engineering via trans unsaturated fatty acids production improves Escherichia coli robustness and production of biorenewables
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 35, May 2016, Pages 105–113
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us