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Improving the performance of solventogenic clostridia by reinforcing the biotin synthetic pathway

Paper ID Volume ID Publish Year Pages File Format Full-Text
31501 44803 2016 8 PDF Available
Title
Improving the performance of solventogenic clostridia by reinforcing the biotin synthetic pathway
Abstract

•First report of engineering robust solventogenic clostridia via reinforcing biotin synthesis.•Expressional fine-tuning of biotin synthetic pathway genes was performed.•The engineered strain featured a high growth rate and productivity in biotin-free medium.•A combined strategy leads to improved co-utilization of hexose and pentose by clostridia.

An efficient production process is important for industrial microorganisms. The cellular efficiency of solventogenic clostridia, a group of anaerobes capable of producing a wealth of bulk chemicals and biofuels, must be improved for competitive commercialization. Here, using Clostridium acetobutylicum, a species of solventogenic clostridia, we revealed that the insufficient biosynthesis of biotin, a pivotal coenzyme for many important biological processes, is a major limiting bottleneck in this anaerobe’s performance. To address this problem, we strengthened the biotin synthesis of C. acetobutylicum by overexpressing four relevant genes involved in biotin transport and biosynthesis. This strategy led to faster growth and improved the titer and productivity of acetone, butanol and ethanol (ABE solvents) of C. acetobutylicum in both biotin-containing and biotin-free media. Expressionally modulating these four genes by modifying the ribosome binding site further promoted cellular performance, achieving ABE solvent titer and productivity as high as 21.9 g/L and 0.30 g/L/h, respectively, in biotin-free medium; these values exceeded those of the wild-type strain by over 30%. More importantly, biotin synthesis reinforcement also conferred improved ability of C. acetobutylicum to use hexose and pentose sugars, further demonstrating the potential of this metabolic-engineering strategy in solventogenic clostridia.

Keywords
Engineering robust clostridia; Biotin synthesis reinforcement; Solvent titer and productivity
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Improving the performance of solventogenic clostridia by reinforcing the biotin synthetic pathway
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 35, May 2016, Pages 121–128
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us