fulltext.study @t Gmail

De novo biosynthesis of Gastrodin in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
31503 44803 2016 10 PDF Available
Title
De novo biosynthesis of Gastrodin in Escherichia coli
Abstract

•An artificial novel biosynthetic pathway for gastrodin was constructed.•Gastrodin was produced from glucose in an E. coli factory for the first time.•Pathway genes were overexpressed to enhance metabolic flux toward aglycon.•Directed evolution was applied to improve catalytic properties of UGT73B6.•∽545 mg/L of gastrodin was produced by the finally engineered E. coli.

Gastrodin, a phenolic glycoside, is the key ingredient of Gastrodia elata, a notable herbal plant that has been used to treat various conditions in oriental countries for centuries. Gastrodin is extensively used clinically for its sedative, hypnotic, anticonvulsive and neuroprotective properties in China. Gastrodin is usually produced by plant extraction or chemical synthesis, which has many disadvantages. Herein, we report unprecedented microbial synthesis of gastrodin via an artificial pathway. A Nocardia carboxylic acid reductase, endogenous alcohol dehydrogenases and a Rhodiola glycosyltransferase UGT73B6 transformed 4-hydroxybenzoic acid, an intermediate of ubiquinone biosynthesis, into gastrodin in Escherichia coli. Pathway genes were overexpressed to enhance metabolic flux toward precursor 4-hydroxybenzyl alcohol. Furthermore, the catalytic properties of the UGT73B6 toward phenolic alcohols were improved through directed evolution. The finally engineered strain produced 545 mg l−1 gastrodin in 48 h. This work creates a new route to produce gastrodin, instead of plant extractions and chemical synthesis.

Keywords
Gastrodin (PubChem CID: 115067); 4-Hydroxybenzyl alcohol (PubChem CID: 125); Tyrosol (PubChem CID: 10393); Salidroside (PubChem CID: 159278); 4-Hydroxybenzoic acid (PubChem CID: 135); 4-Methylumbelliferone (PubChem CID: 5280567); 4-Methylumbelliferyl β-D-
First Page Preview
De novo biosynthesis of Gastrodin in Escherichia coli
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 35, May 2016, Pages 138–147
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us