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A carbon sink pathway increases carbon productivity in cyanobacteria

Paper ID Volume ID Publish Year Pages File Format Full-Text
31516 44807 2015 7 PDF Available
Title
A carbon sink pathway increases carbon productivity in cyanobacteria
Abstract

•A carbon sink pathway increased total carbon productivity in cyanobacteria.•This method led to 1.8-fold increase in total carbon yield.•Specific 2,3-butanediol production increased 2.4-fold.•Partitioning to the product beyond 30% led to a steep decline in total carbon yield.

The burning of fossil reserves, and subsequent release of carbon into the atmosphere is depleting the supply of carbon-based molecules used for synthetic materials including plastics, oils, medicines, and glues. To provide for future society, innovations are needed for the conversion of waste carbon (CO2) into organic carbon useful for materials. Chemical production directly from photosynthesis is a nascent technology, with great promise for capture of CO2 using sunlight. To improve low yields, it has been proposed that photosynthetic capacity can be increased by a relaxation of bottlenecks inherent to growth. The limits of carbon partitioning away from growth within the cell and the effect of partitioning on carbon fixation are not well known. Here we show that expressing genes in a pathway between carbon fixation and pyruvate increases partitioning to 2,3-butanediol (23BD) and leads to a 1.8-fold increase in total carbon yield in the cyanobacterium Synechococcus elongatus PCC 7942. Specific 2,3-butanediol production increases 2.4-fold. As partitioning increases beyond 30%, it leads to a steep decline in total carbon yield. The data suggests a local maximum for carbon partitioning from the Calvin Benson cycle that is scalable with light intensity.

Keywords
Cyanobacteria; Metabolic engineering; 2,3-Butanediol; CO2 fixation; Photosynthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 29, May 2015, Pages 106–112
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us