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Engineering the bacterial shapes for enhanced inclusion bodies accumulation

Paper ID Volume ID Publish Year Pages File Format Full-Text
31529 44807 2015 11 PDF Available
Title
Engineering the bacterial shapes for enhanced inclusion bodies accumulation
Abstract

•Deletion on actin-like protein MreB increased bacterial cell sizes.•Larger spherical E. coli cells have larger intracellular volumes for inclusion body storages.•Elongated E. coli formed by over expression of mreB and sulA stabilized filamentary shapes.•109% PHB accumulation increase was achieved by morphology manipulated E. coli.

Many bacteria can accumulate inclusion bodies such as sulfur, polyphosphate, glycogen, proteins or polyhydroxyalkanoates. To exploit bacteria as factories for effective production of inclusion bodies, a larger intracellular space is needed for more inclusion body accumulation. In this study, polyhydroxybutyrate (PHB) was investigated as an inclusion bodies representative to be accumulated by Escherichia coli JM109SG. Various approaches were taken to increase the bacterial cell sizes including deletion on actin-like protein gene mreB, weak expression of mreB in mreB deletion mutant, and weak expression of mreB in mreB deletion mutant under inducible expression of SulA, the inhibitor of division ring protein FtsZ. All of the methods resulted in different levels of increases in bacterial sizes and PHB granules accumulation. Remarkably, an increase of over 100% PHB accumulation was observed in recombinant E. coli overexpressing mreB in an mreB deletion mutant under inducible expression of FtsZ inhibiting protein SulA. The molecular mechanism of enlarged bacterial size was found to be directly relate to weakened cytoskeleton which was the result of broken skeleton helix.

Keywords
Cell morphology; PHB; mreB; Escherichia coli; Cell size; Inclusion bodies
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Engineering the bacterial shapes for enhanced inclusion bodies accumulation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 29, May 2015, Pages 227–237
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us