fulltext.study @t Gmail

Mathematical modeling of functionalized-microsphere based assays for rapid DNA detection: From sample preparation to results

Paper ID Volume ID Publish Year Pages File Format Full-Text
3153 153 2014 11 PDF Available
Title
Mathematical modeling of functionalized-microsphere based assays for rapid DNA detection: From sample preparation to results
Abstract

•We present a model for biomolecule detection by microspheres conjugation.•The model accounts for sample preparation in a lysis microreactor.•The model is applied to a diagnostic system based on the HRP enzyme.•The model provides quantitative estimates regarding the system sensitivity.•The HRP-based diagnostic is shown to be rapid with very high sensitivity.

Functionalized microspheres are frequently used in the detection of biomolecules. A fundamental understanding of the mechanisms involved in enzymatic assays is required to estimate a method's utility. The mathematical methodology is illustrated through a theoretical analysis to assess the performance of a diagnostic method based on the horseradish peroxidase catalyzed reaction to detect pathogens in clinical specimens. The first part of the analysis focuses on the collection of target DNA molecules onto a functionalized fiber in a lysis micro-reactor (LMR). Expressions are derived for hybridization rates that include convective transport. In the next step the fiber is contacted with functionalized chitosan microspheres containing horse radish peroxidase. Chitosan microspheres are conjugated to the fiber with the target DNA acting as a unique tether. The final step is the release of chitosan microspheres in a chromogenic substrate and monitoring absorption changes. The analysis provides estimates of the method sensitivity and processing time and we show that DNA with a concentration as low as 10−4 copies per milliliter can be detected in less than 30 min. The modeling methodology presented can readily be extended to similar enzymatic, microsphere-based assays for quantitative purposes and feasibility studies.

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
PCR, polymerase chain reaction; MB, molecular beacons; HRP, Horse radish peroxidase; LMR, Lysis micro-reactor; CM, Chitosan Microsphere; TMB, tetra-methyl-benzidine; CCD, charge-coupled deviceBiomedical; Diffusion-reaction; Diagnostics; Dynamic modeling;
First Page Preview
Mathematical modeling of functionalized-microsphere based assays for rapid DNA detection: From sample preparation to results
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 88, 15 July 2014, Pages 50–60
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us