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Enhanced levels of S-linalool by metabolic engineering of the terpenoid pathway in spike lavender leaves

Paper ID Volume ID Publish Year Pages File Format Full-Text
31544 44810 2014 9 PDF Available
Title
Enhanced levels of S-linalool by metabolic engineering of the terpenoid pathway in spike lavender leaves
Abstract

•Transgenic spike lavender plants expressing a linalool synthase gene were generated.•Transgenic plants produced up to a 1000% linalool increase in youngest leaves.•Leaf linalool content directly correlated with transgene transcript levels.

Transgenic Lavandula latifolia plants overexpressing the linalool synthase (LIS) gene from Clarkia breweri, encoding the LIS enzyme that catalyzes the synthesis of linalool were generated. Most of these plants increased significantly their linalool content as compared to controls, especially in the youngest leaves, where a linalool increase up to a 1000% was observed. The phenotype of increased linalool content observed in young leaves was maintained in those T1 progenies that inherit the LIS transgene, although this phenotype was less evident in the flower essential oil. Cross-pollination of transgenic spike lavender plants allowed the generation of double transgenic plants containing the DXS (1-deoxy-d-xylulose-5-P synthase), coding for the first enzyme of the methyl-d-erythritol-4-phosphate pathway, and LIS genes. Both essential oil yield and linalool content in double DXS-LIS transgenic plants were lower than that of their parentals, which could be due to co-suppression effects linked to the structures of the constructs used.

Keywords
Essential oils; Lavandula latifolia; Linalool synthase; Metabolic engineering; Monoterpenes
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 23, May 2014, Pages 136–144
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
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Online Support
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