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Combinatorial biosynthesis of plant-specific coumarins in bacteria

Paper ID Volume ID Publish Year Pages File Format Full-Text
31557 44815 2013 9 PDF Available
Title
Combinatorial biosynthesis of plant-specific coumarins in bacteria
Abstract

•Design of artificial biosynthetic pathways of plant coumarins in Escherichia coli.•Obviation of plant cytochrome P450 enzymes with a promiscuous bacterial hydroxylase.•Bioconversion of inexpensive phenylpropanoid acids into simple coumarins.•De novo biosynthesis of umbelliferone and scopoletin.

Coumarins are plant secondary metabolites that have demonstrated a variety of important therapeutic properties, such as antibacterial, anti-inflammatory, and anti-coagulant effects, as well as anti-cancer and anti-AIDS activities. However, knowledge regarding their biosynthesis is relatively limited even for the simplest coumarin molecule, which serves as the gateway molecule to many pharmaceutically important coumarin derivatives. Here we reported the design and validation of artificial pathways leading to the biosynthesis of plant-specific simple coumarins in bacteria. First, Escherichia coli strains were engineered to convert inexpensive phenylpropanoid acid precursors, 4-coumarate and ferulate to simple coumarins, umbelliferone (4.3 mg/L) and scopoletin (27.8 mg/L), respectively. Furthermore, we assembled the complete artificial pathways in E. coli and achieved de novo biosynthesis of umbelliferone and scopoletin without addition of precursors. This study lays the foundation for microbial production of more diverse coumarin compounds.

Keywords
Coumarin; Scopoletin; Umbelliferone; Plant metabolites; Combinatorial biosynthesis
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 18, July 2013, Pages 69–77
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us