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A new method for the rapid separation of magnetized yeast in sparkling wine

Paper ID Volume ID Publish Year Pages File Format Full-Text
3156 153 2014 8 PDF Available
Title
A new method for the rapid separation of magnetized yeast in sparkling wine
Abstract

•Novel method for the rapid magnetic separation of yeast cells from sparkling wine.•Synthesis of superparamagnetic maghemite nanoparticles with a positive surface charge.•Absorption of positively charged nanoparticles onto negatively charged yeast cells.•Influence of magnetic nanoparticles on the yeast metabolism and process kinetics.•Separation of the “magnetized” yeast biomass in a magnetic field.

A novel method for the rapid magnetic separation of wine yeast cells from sparkling wine was developed. The cells were made responsive to a magnetic field by the absorption of superparamagnetic nanoparticles of iron oxide maghemite (γ-Fe2O3) coated with a thin layer of silica and grafted with (aminoethylamino)propylmethyldimethoxysilane (APMS). The terminal amino groups of the APMS molecules provide a positive charge on the nanoparticles’ surfaces and promote their electrostatic absorption onto the negatively charged surfaces of the cells. The optimal mass ratio between the magnetic nanoparticles and the wine yeast was determined to be 1:10. The separation of the “magnetized” biomass in the magnetic field as well as the influence of the magnetic nanoparticles on the yeast metabolism was studied. Scanning and transmission electron microscopy showed that the magnetic nanoparticles remained fixed at the microbial cell surfaces, even after fermentation. The results of the chemical analysis demonstrated that besides the faster microbial kinetics there were no negative influences on the cell metabolism. The same results were confirmed in sensorial analyses of the sparkling wine. The separation of the magnetized waste biomass in the bottle neck using relatively weak magnetic-field gradient can be successfully completed in approximately 15 min.

Keywords
Fermentation; Bubbles; Wine yeast; Optimisation of biomass separation; Nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 88, 15 July 2014, Pages 77–84
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us