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Metabolic engineering of Escherichia coli for the production of 1-propanol

Paper ID Volume ID Publish Year Pages File Format Full-Text
31561 44819 2012 10 PDF Available
Title
Metabolic engineering of Escherichia coli for the production of 1-propanol
Abstract

An engineered Escherichia coli strain that produces 1-propanol under aerobic condition was developed based on an l-threonine-overproducing E. coli strain. First, a feedback resistant ilvA gene encoding threonine dehydratase was introduced and the competing metabolic pathway genes were deleted. Further engineering was performed by overexpressing the cimA gene encoding citramalate synthase and the ackA gene encoding acetate kinase A/propionate kinase II, introducing a modified adhE gene encoding an aerobically functional AdhE, and by deleting the rpoS gene encoding the stationary phase sigma factor. Fed-batch culture of the final engineered strain harboring pBRthrABC-tac-cimA-tac-ackA and pTacDA-tac-adhEmut allowed production of 10.8 g L−1 of 1-propanol with the yield and productivity of 0.107 g g−1 and 0.144 g L−1 h−1, respectively, from 100 g L−1 of glucose, and 10.3 g L−1 of 1-propanol with the yield and productivity of 0.259 g g−1 and 0.083 g L−1 h−1, respectively, from 40 g L−1 glycerol.

► 1-propanol producing E. coli strain was developed by metabolic engineering. ► Use of aerobically functional AdhE allowed 1-propanol production under aerobic condition. ► Overexpression of cimA and deletion of rpoS increased 1-propanol production. ► The final engineered strain produced more than 10 g L−1 of 1-propanol from glucose or glycerol.

Keywords
Escherichia coli; 1-propanol; l-threonine; 2-ketobutyrate; In silico metabolic flux analysis; Glycerol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 14, Issue 5, September 2012, Pages 477–486
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us