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Harnessing recombination to speed adaptive evolution in Escherichia coli

Paper ID Volume ID Publish Year Pages File Format Full-Text
31562 44819 2012 9 PDF Available
Title
Harnessing recombination to speed adaptive evolution in Escherichia coli
Abstract

Evolutionary engineering typically involves asexual propagation of a strain to improve a desired phenotype. However, asexual populations suffer from extensive clonal interference, a phenomenon where distinct lineages of beneficial clones compete and are often lost from the population given sufficient time. Improved adaptive mutants can likely be generated by genetic exchange between lineages, thereby reducing clonal interference. We present a system that allows continuous in situ recombination by using an Esherichia coli F-based conjugation system lacking surface exclusion. Evolution experiments revealed that Hfr-mediated recombination significantly speeds adaptation in certain circumstances. These results show that our system is stable, effective, and suitable for use in evolutionary engineering applications.

► E. coli system that facilitates interclonal genetic exchange in situ during adaptive evolution. ► Expedite the process of adaptive evolution for complex phenotypes. ► Broadly applicable for evolutionary engineering for strain development.

Keywords
Evolutionary engineering; Recombination; Clonal interference; Complex phenotypes
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Harnessing recombination to speed adaptive evolution in Escherichia coli
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 14, Issue 5, September 2012, Pages 487–495
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us