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Urease expression in a Thermoanaerobacterium saccharolyticum ethanologen allows high titer ethanol production

Paper ID Volume ID Publish Year Pages File Format Full-Text
31567 44819 2012 5 PDF Available
Title
Urease expression in a Thermoanaerobacterium saccharolyticum ethanologen allows high titer ethanol production
Abstract

Genes encoding the enzyme urease were integrated in a Thermoanaerobacterium saccharolyticum ethanologen. The engineered strain hydrolyzed urea, as evidenced by increased cellular growth and elevated final pH in urea minimal medium and urease activity in cell free extracts. Interestingly, replacement of ammonium salts with urea resulted in production of 54 g/L ethanol, one of the highest titers reported for Thermoanaerobacterium. The observed increase in ethanol titer may result from reduced pH, salt, and osmolality stresses during fermentation. Urea utilization is attractive for industrial scale fermentation, where pH control is technically challenging and increased ethanol titer is desirable.

► Genes encoding urease were integrated in a T. saccharolyticum ethanologen. ► Replacement of ammonium salts with urea resulted in production of 54 g/L ethanol. ► Urea utilization is attractive for industrial scale fermentation.

Keywords
Biofuels; Thermophiles; Ethanol
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Urease expression in a Thermoanaerobacterium saccharolyticum ethanologen allows high titer ethanol production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 14, Issue 5, September 2012, Pages 528–532
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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