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Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform

Paper ID Volume ID Publish Year Pages File Format Full-Text
31579 44821 2012 8 PDF Available
Title
Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform
Abstract

Epidemiological studies have shown that consumption of cruciferous vegetables, such as, broccoli and cabbages, is associated with a reduced risk of developing cancer. This phenomenon has been attributed to specific glucosinolates among the ∼30 glucosinolates that are typically present as natural products characteristic of cruciferous plants. Accordingly, there has been a strong interest to produce these compounds in microbial cell factories as it will allow production of selected beneficial glucosinolates. We have developed a versatile platform for stable expression of multi-gene pathways in the yeast, Saccharomyces cerevisiae. Introduction of the seven-step pathway of indolylglucosinolate from Arabidopsis thaliana to yeast resulted in the first successful production of glucosinolates in a microbial host. The production of indolylglucosinolate was further optimized by substituting supporting endogenous yeast activities with plant-derived enzymes. Production of indolylglucosinolate serves as a proof-of-concept for our expression platform, and provides a basis for large-scale microbial production of specific glucosinolates for the benefit of human health.

► Expression platform tailored for stable expression of multiple genes was developed. ► Used for the transfer of the seven step pathway of indolylglucosinolate into Saccharomyces cerevisiae. ► First example of glucosinolate production in a microbial host. ► Substitution of supporting Saccharomyces cerevisiae enzymes with plant enzymes increased production.

Keywords
IG, indol-3-methyl glucosinolate; YPD, rich yeast extract based medium containing glucose; YPgal, rich yeast extract based medium containing galactose; GSH, glutathione; YFG, your favorite gene; 5-FOA, 5-fluoroorotic acidGlucosinolates; Yeast expression p
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Microbial production of indolylglucosinolate through engineering of a multi-gene pathway in a versatile yeast expression platform
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Publisher
Database: Elsevier - ScienceDirect
Journal: Metabolic Engineering - Volume 14, Issue 2, March 2012, Pages 104–111
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us